Translational_Unit

Part:BBa_K112232:Design

Designed by: Bing Xia   Group: iGEM08_UC_Berkeley   (2008-10-21)


rbs.barstar!


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

Construction of {rbs.barstar!} Biobrick Part K112232
PCR mea035/mea034 on Bacillus amyloliquefaciens gen          (320 bp, EcoRI/BamHI)
Sub into pBca1256                                            (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112232
----------------------------------------
mea035           Forward Biobricking of {rbs.barstar!}  
cgataGAATTCatgAGATCTcataagaaaggagccgcacatg
mea034           Reverse Biobricking of {rbs.barstar!}  
cgttaGGATCCttaagaaagtatgatggtgatgtcgcagcc

References